solution

In the chart below, fill in the genotype of E. coli prior to transformation and after transformation. What was the purpose of transferring the +DNA and -DNA tubes from ice, to hot water, to ice again? ____ Why were the vials incubated for 10 minutes in LB broth rather than transferring their contents directly to the plates? ____ What information is provided by the LB/-DNA plate? ____ Obviously, transformation could only occur if the pGLO plasmid was introduced into the solution. Which plate(s) exhibit transformation? ____ What information is provided by the LB/amp/-DNA plate? ____ Why does the LB/amp/ara/+DNA plate fluoresce when the LB/amp/+DNA plate does not ? ____

In the chart below, fill in the genotype of E. coli prior to transformation and after...

 
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